The Mutagenesis Proteins UmuD′ and UmuC Prevent Lethal Frameshifts While Increasing Base Substitution Mutations
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چکیده
منابع مشابه
Regulation of SOS mutagenesis by proteolysis ( UmuD / D ' / UmuC / Lon / ClpXP / protein - protein interactions )
DNA damage-inducible mutagenesis in Escherichia coli is largely dependent upon the activity of the UmuD (UmuD') and UmuC proteins. The intracellular level of these proteins is tightly regulated at both the transcriptional and the posttranslational levels. Such regulation presumably allows cells to deal with DNA damage via error-free repair pathways before being committed to error-prone pathways...
متن کاملTargeting of the UmuD, UmuD', and MucA' mutagenesis proteins to DNA by RecA protein.
In addition to its critical role in genetic recombination, the Escherichia coli RecA protein plays a pivotal role in SOS-induced mutagenesis. This role can be separated genetically into three steps: (i) depression of the SOS regulon by mediating the posttranslational cleavage of the LexA repressor, (ii) activation of UmuD'-like proteins by mediating cleavage of the UmuD-like proteins, and (iii)...
متن کاملChapter 773 UmuD and UmuD′ Proteins
Escherichia coli. Cell 29, 11 22. [17] Friedberg, E.C., Walker, G.C., Siede, W., Wood, R.D., Schultz, R.A., Ellenberger, T. (2005). DNA Repair and Mutagenesis, 2nd edn, Washington, DC: American Society of Microbiology Press. [18] Sassanfar, M., Roberts, J.W. (1990). Nature of the SOS-inducing signal in Escherichia coli. The involvement of DNA replication. J. Mol. Biol. 212, 79 96. [19] Ptashne,...
متن کاملThe Roles of UmuD in Regulating Mutagenesis
All organisms are subject to DNA damage from both endogenous and environmental sources. DNA damage that is not fully repaired can lead to mutations. Mutagenesis is now understood to be an active process, in part facilitated by lower-fidelity DNA polymerases that replicate DNA in an error-prone manner. Y-family DNA polymerases, found throughout all domains of life, are characterized by their low...
متن کاملThe beta subunit sliding DNA clamp is responsible for unassisted mutagenic translesion replication by DNA polymerase III holoenzyme.
The replication of damaged nucleotides that have escaped DNA repair leads to the formation of mutations caused by misincorporation opposite the lesion. In Escherichia coli, this process is under tight regulation of the SOS stress response and is carried out by DNA polymerase III in a process that involves also the RecA, UmuD' and UmuC proteins. We have shown that DNA polymerase III holoenzyme i...
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ژورنال
عنوان ژورنال: Molecular Cell
سال: 1998
ISSN: 1097-2765
DOI: 10.1016/s1097-2765(00)80129-x